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ORIGINAL ARTICLE
CYTOKERATIN 16 EXPRESSION DOES NOT INDICATE
CHOLESTEATOMA AGGRESSIVENESS
1
Department of Otorhinolaryngology Head and Neck Surgery and Department of Pathology, Ain-Shams
University Faculty of Medicine, Cairo, Egypt
Publication date: 2012-09-30
Corresponding author
Badr Eldin Mostafa
Badr Eldin Mostafa, Professor of Otorhinolaryngology, Faculty of Medicine, Ain-Shams University, 48 Ibn El Nafees Street, 11371 Nasr City, Cairo, Egypt, e-mail: bemostafa@med.asu.edu.eg
Badr Eldin Mostafa, Professor of Otorhinolaryngology, Faculty of Medicine, Ain-Shams University, 48 Ibn El Nafees Street, 11371 Nasr City, Cairo, Egypt, e-mail: bemostafa@med.asu.edu.eg
J Hear Sci 2012;2(3):50–54
KEYWORDS
ABSTRACT
Aims:
Cholesteatoma is characterised by a squamous epithelium in the middle ear and aggressive destructive behavior. A hyperproliferative epithelium also typically has an overexpression of cytokeratin 16 (CK16). The aim of this study was to compare CK16 expression in cholesteatoma epithelium and the epithelium of simple dry perforations.
Methods:
Samples were obtained from 60 patients: 30 with cholesteatoma and 30 with simple perforations. The epithelium was immunohistochemically stained for CK16. Correlations were made between the clinical picture and the absence or presence of complications in both groups.
Results:
Immunohistochemical staining for CK16 was positive in 45% of specimens taken from the edge of simple perforations and in 51% of specimens taken from the cholesteatoma matrix. The difference was not statistically significant.
Conclusion:
Two implications of our findings may be suggested: in cases of cholesteatoma the change in CK expression from normal epithelium is not the only factor which makes cholesteatoma aggressive; alternatively, in normal tissue the edge of a simple perforation is not as stable as is thought so that at least 45% of perforations can show hyperproliferative activity. It is therefore unclear whether hyperproliferative activity in either cholesteatoma or a simple perforation is a marker of instability or of attempts at healing.
Cholesteatoma is characterised by a squamous epithelium in the middle ear and aggressive destructive behavior. A hyperproliferative epithelium also typically has an overexpression of cytokeratin 16 (CK16). The aim of this study was to compare CK16 expression in cholesteatoma epithelium and the epithelium of simple dry perforations.
Methods:
Samples were obtained from 60 patients: 30 with cholesteatoma and 30 with simple perforations. The epithelium was immunohistochemically stained for CK16. Correlations were made between the clinical picture and the absence or presence of complications in both groups.
Results:
Immunohistochemical staining for CK16 was positive in 45% of specimens taken from the edge of simple perforations and in 51% of specimens taken from the cholesteatoma matrix. The difference was not statistically significant.
Conclusion:
Two implications of our findings may be suggested: in cases of cholesteatoma the change in CK expression from normal epithelium is not the only factor which makes cholesteatoma aggressive; alternatively, in normal tissue the edge of a simple perforation is not as stable as is thought so that at least 45% of perforations can show hyperproliferative activity. It is therefore unclear whether hyperproliferative activity in either cholesteatoma or a simple perforation is a marker of instability or of attempts at healing.
REFERENCES (8)
1.
Kim HJ, Tinling SP, Chole RA: Expression patterns of cytokeratins in cholesteatoma: evidence of increased migration and proliferation. J Korean Med Sci, 2002; 17(3): 381–88.
2.
Moll R, Franke WW, Schiller DL: The catalog of human CK: patterns of expression in normal epithelia, tumours and cultured cells. Cell, 1982; 31: 11–24.
3.
Vennix P, Kuijpers W, Peters T et al: Keratinocyte differentiation in acquired cholesteatoma and perforated tympanic membranes. Arch Otorhinolaryngol Head Neck Surg, 1996; 122: 825–32.
4.
Olszewska E, Sudhoff H: Comparative cytokeratin distribution patterns in cholesteatoma epithelium. Histol Histopathol, 2007; 22(1): 37–42.
5.
Sasaki H, Huang CC: Expression of cytokeratins 13 and 16 in middle ear cholesteatoma. Otolaryngol Head Neck Surg, 1994; 110(3): 310–17.
6.
Lee RJ, Sidey C, Narula AA, James RF: The nature of the epithelium in acquired cholesteatoma: Part 3 – Cytokeratin patterns in aural epithelial and cholesteatoma cells grown in cell culture. Clin Otolaryngol Allied Sci, 1994; 19(6): 516–20.
7.
Kim HJ, Tinling SP, Chole RA: Expression patterns of cytokeratins in retraction pocket cholesteatomas. Laryngoscope, 2001; 111(6): 1032–36.
8.
Bujia J, Schilling V, Holly A et al: Hyperproliferation-associated keratin expression in human middle ear cholesteatoma. Acta Otorhinolaryngol (Stockh), 1993; 113: 364–68.
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